Dual Functions, Clamp Opening and Primer-Template Recognition, Define a Key Clamp Loader Subunit
نویسندگان
چکیده
منابع مشابه
Dual functions, clamp opening and primer-template recognition, define a key clamp loader subunit.
Clamp loader proteins catalyze assembly of circular sliding clamps on DNA to enable processive DNA replication. During the reaction, the clamp loader binds primer-template DNA and positions it in the center of a clamp to form a topological link between the two. Clamp loaders are multi-protein complexes, such as the five protein Escherichia coli, Saccharomyces cerevisiae, and human clamp loaders...
متن کاملThe Mechanism of ATP-Dependent Primer-Template Recognition by a Clamp Loader Complex
Clamp loaders load sliding clamps onto primer-template DNA. The structure of the E. coli clamp loader bound to DNA reveals the formation of an ATP-dependent spiral of ATPase domains that tracks only the template strand, allowing recognition of both RNA and DNA primers. Unlike hexameric helicases, in which DNA translocation requires distinct conformations of the ATPase domains, the clamp loader ...
متن کاملConserved residues in the δ subunit help the E. coli clamp loader, γ complex, target primer-template DNA for clamp assembly
The Escherichia coli clamp loader, gamma complex (gamma(3)deltadelta'lambdapsi), catalyzes ATP-driven assembly of beta clamps onto primer-template DNA (p/tDNA), enabling processive replication. The mechanism by which gamma complex targets p/tDNA for clamp assembly is not resolved. According to previous studies, charged/polar amino acids inside the clamp loader chamber interact with the double-s...
متن کاملConserved residues in the delta subunit help the E. coli clamp loader, gamma complex, target primer-template DNA for clamp assembly
The Escherichia coli clamp loader, c complex (c3dd’kc), catalyzes ATP-driven assembly of b clamps onto primer-template DNA (p/tDNA), enabling processive replication. The mechanism by which c complex targets p/tDNA for clamp assembly is not resolved. According to previous studies, charged/polar amino acids inside the clamp loader chamber interact with the double-stranded (ds) portion of p/tDNA. ...
متن کاملHow a DNA polymerase clamp loader opens a sliding clamp.
Processive chromosomal replication relies on sliding DNA clamps, which are loaded onto DNA by pentameric clamp loader complexes belonging to the AAA+ family of adenosine triphosphatases (ATPases). We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template DNA. The clamp loader traps a spiral conformation of the ope...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2004
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2004.07.097